Mark S. Ladinsky, J. Richard McIntosh, David N. Mastronarde, Kathryn E. Howell, L. Andrew Staehelin (2011) CIL:24477, Rattus norvegicus, epithelial cell. CIL. Dataset

This image is from a tomogram through part of a Golgi ribbon from a normal rat kidney cell, generated using high voltage electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The sample was tilted at angles of 1.5° to obtain the series of images in this set, and used to obtain 3-dimensional reconstructions of the Golgi apparatus and contributed to findings reported in Ladinsky et al. (1999) Golgi structure in three dimensions: Functional insights from the normal rat kidney cell. J. Cell Biol. 144 (6) 1135-1149.

该图像源自正常大鼠肾脏细胞部分高尔基带的高电压电子显微镜断层扫描，经15°C孵育以阻断高尔基复合体中的物质运输。温度阻断后，在三个最外侧的囊泡上出现了大型膨胀区。为获取本系列图像，样本以1.5°的倾斜角度拍摄，并用于高尔基器的三维重建，这些发现有助于Ladinsky等（1999年）在《细胞生物学杂志》上发表的论文《三维高尔基结构：正常大鼠肾脏细胞的功能见解》中的相关报道。J. Cell Biol. 144 (6) 1135-1149。