An Amygdalar Local Oscillator Coordinates Cellular and Behavioral Rhythms

Purpose: scRNA-Seq and snRNA were performed to characterize mWake-expressing neurons in the lateral amygdala Overall design: The lateral amygdalae were collected from 7-8 wk old male mWake(Cre/+) mice at ZT0-2 using a previously published protocol. Briefly, the coronal plane (350 um) containing the LA was sliced using a vibratome in bubbling aCSF. LA were dissected into Hibernate-A media with a 2% B-27 and GlutaMAX supplement (0.5 mM final concentration). Both LA from 2 mice were used for scRNA-Seq analysis, which was repeated with 2 additional mice. This procedure was also performed for snRNA-Seq analysis (in total, 8 mice were used).