Combinatorial and assembly analysis of different STNV move sets.

For each of the different move sets, we computed the number of unique pseudo-Hamiltonian paths (Npath) and performed a stochastic assembly simulation with 2000 copies of an RNA containing 30 PSs with identical affinities (CP-PS binding energy set to ΔGps = −9 kcal M−1) and determined the number of capsids assembled at equilibrium (Ncap). Data for the number of pseudo-Hamiltonian paths (Npath) was obtained using our in house depth-first search algorithm. The number of capsids assembled was rounded to the nearest 10 and move sets which generated a negligible but non-zero number of capsids have been marked as “