Data for: Identification of bacterial isolates from a public hospital in Australia using complexity-reduced genotyping

These compressed directories contain fastA files of complexity-reduced genotyping by sequencing data of bacterial isolates from a public hospital in Australia. Sequencing data of a total of 165 bacterial isolates are included in these data sets of the following species: Enterococcus faecium, Staphylococcus aureus, Enterobacter cloacae complex, Citrobacter freundii, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter asburiae, Escherichia coli, Morganella morganii, Citrobacter amalonaticus, Enterobacter aerogenes, Enterococcus faecalis, Hafnia alvei, Providencia rettgeri and Serratia marcescens. Additionally, this dataset includes sequencing results of genomic DNA of E. coli O157 (EDL 933) IRMM449 Sigma-Aldrich certified reference. All bacterial isolates were processed with the following combination of restriction enzymes: PstI with MseI, PstI with HpaII and MseI with HpaII. Some samples contain technical replicates and the certified reference contains six technical replicates. Each directory contains a Microsoft Excel Comma Separated Values File with bacterial isolate information, including an internal unique identity number (TargetID), the sample name (Genotype), material used for DNA extraction (Tissue), complexity-reduced genotyping method (Comment), and other details produced after sequencing (e.g. extract plate barcode, extract well, flowcell ID, flowcell lane). For a detailed description of how this data was obtained, please refer to the article "Identification of bacterial isolates from a public hospital in Australia using complexity-reduced genotyping (2019) Berenice Talamantes-Becerra, Jason Carling, Karina Kennedy, Michelle E. Gahan, Arthur Georges. Journal of Microbiological Methods, Volume 160, May 2019, Pages 11-19. https://doi.org/10.1016/j.mimet.2019.03.016

本数据集包含澳大利亚一家公立医院细菌分离株的简化基因分型测序数据压缩目录。共计包含165株细菌分离株的测序数据，涉及以下物种：粪肠球菌、金黄色葡萄球菌、肠杆菌科复杂群、弗氏柠檬酸杆菌、肺炎克雷伯菌、阴沟肠杆菌、产气肠杆菌、大肠杆菌、摩根菌、恶臭假单胞菌、恶臭假单胞菌、粪肠球菌、产气克雷伯菌、阴沟肠杆菌、大肠杆菌O157（EDL 933）IRMM449 Sigma-Aldrich认证参考菌株的基因组DNA测序结果。所有细菌分离株均使用以下限制性内切酶组合进行处理：PstI与MseI、PstI与HpaII以及MseI与HpaII。部分样本包含技术重复，而认证参考菌株包含六个技术重复。每个目录均包含一个Microsoft Excel逗号分隔值文件，其中包含细菌分离株信息，包括内部唯一标识符（TargetID）、样本名称（基因型）、用于DNA提取的材料（组织）、简化基因分型方法（注释）以及测序后产生的其他详细信息（例如提取板条形码、提取孔、流动单元ID、流动单元通道）。有关如何获取这些数据的详细描述，请参阅文章《利用简化基因分型鉴定澳大利亚公立医院细菌分离株（2019）Berenice Talamantes-Becerra, Jason Carling, Karina Kennedy, Michelle E. Gahan, Arthur Georges. 微生物方法杂志，第160卷，2019年5月，第11-19页。https://doi.org/10.1016/j.mimet.2019.03.016》