Programmable liposome organization via DNA origami templates

Liposomes are essential vehicles for membrane protein reconstitution and
drug delivery, making them vital tools in both in vivo and in vitro
studies. However, the lack of robust techniques for the precise
arrangement of these synthetic vesicles limits their potential
applications. Here, we present a modular polymerization platform based on
square DNA origami to template the formation and organization of
liposomes. By programming the sequence, number, position, chirality, and
flexibility of sticky ends on each square, we assemble uniformly sized
liposomes into diverse two-dimensional (2D) arrays, as well as finite
lattices and rings. Additionally, we demonstrate stepwise assembly and
targeted disassembly, enabling dynamic structural control. These complex
liposome architectures represent a significant advancement in the fields
of biotechnology, nanotechnology, and bottom-up biology.