The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

We hypothesize that Ibd1 coordinates the recruitment of several chromatin remodeling complexes to promote transcription of highly expressed genes in T.thermophila. We developed a ChIP-seq protocol including a heuristic method (in preparation paper, Ponce & Saettone, et al., 2017) to call peaks present in genic and intergenic regions specifically. We used the obtained data from the 2 NGS biological replicas, each of which had an INPUT and an Immunoprecipitated (IP) sample. Overall design: Examination of 2 biological replicates of Ibd1 (BRD-containing protein) during vegetative growth.