Supplemental Table S3
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Primers used to amplify wild-type genes corresponding to transposon mutants and clone them into pOS1-Plgt with splicing overlap extension (SOE)-PCR or HiFi/Gibson assembly. The annealing portion is bolded. Tms were calculated for NEB Q5 HF polymerase (https://tmcalculator.neb.com/#!/batch). fmtC primers were used to amplify fmtC from USA300 and NRS209.
创建时间：
2020-12-10
