Spatial cartography of human thymus enables the geopositioning of rare mimetic thymic epithelial cells

In this study, we applied the Stereo-seq spatial transcriptomics method to establish a high-resolution spatial atlas for the human fetal and pediatric thymus. We profiled fetal thymic tissues in the second trimester at 13, 14, 17, and 18 weeks post-conception, and in pediatric ages of 7 weeks and 2, 5, and 6 years. A01785D6.tissue.gef Human fetal: 13w spatial transcriptomics count data A01785C6.tissue.gef Human fetal: 18w spatial transcriptomics count data C02931A1B3.tissue.gef Human pediatric: 7w spatial transcriptomics count data B01809D6.tissue.gef Human fetal: 14w spatial transcriptomics count data D02672A5.tissue.gef Human pediatric: 5y spatial transcriptomics count data SS200000128BR_E1.gef Human fetal: 17w spatial transcriptomics count data C02935F5.tissue.gef Human pediatric: 2y spatial transcriptomics count data C04297D1.tissue.gef Human pediatric: 6y spatial transcriptomics count data B01809E3.tissue.gef Mouse: 1-month spatial transcriptomics count data SS200001298TL_F5.tissue.gef Mouse: 3-month spatial transcriptomics count data Data_manu_20250826.rds Integrated ScRNA-seq data Seurat R object shinyApp_scRNA.zip Web App files for viewing integrated scRNA-seq data in ShinyApp Spatial_17week_fetal_bin50_ShinyApp.zip Web App files for viewing integrated spatial fetal 17w data in ShinyApp Spatial_shinyApp_Feta_14w_bin50_select.zip Web App files for viewing integrated spatial fetal 14w data in ShinyApp A03994E6.tissue.gef Human pediatric: 7w spatial transcriptomics count data (Cite-Stereoseq) A03994E6.protein.tissue.gef Human pediatric: 7w spatial protein count data (Cite-Stereoseq)