Transcriptomic analysis of murine microglia from embryonic development to adulthood

Microarray analysis of murine microglia from different stages of development was performed. Results showed that different phases of microglia development had different group of genes up-regulated for specific functions. RNA was extracted from microglia/progenitors from E10.5 embryos to adult mice. Gene expression analysis was done to observe DEGs and differential pathway analysis.本研究针对不同发育阶段的小鼠小胶质细胞（microglia）开展基因芯片分析。结果表明，小胶质细胞发育的不同阶段，其为实现特定功能而上调的基因群存在显著差异。实验从胚胎发育第10.5天（E10.5）的胚胎直至成年小鼠的小胶质细胞/祖细胞中提取RNA，通过基因表达分析以检测差异表达基因（Differentially Expressed Genes，DEGs），并完成差异通路分析。