aITC was carried out at 28°C in 10 mM Tris-HCl, pH 7.6, 150 mM NaCl. bThe neutralization IC50 values for Fab8066, taken from ref. [22], are provided for comparison. The KD for binding to 5-helix, also

NIAID Data Ecosystem40

Summary of the binding affinities of human NGF wild type and the mutants for the MAb anti-NGF: R&D (MAB 256) and αD11[29] in Surface Plasmon Resonance binding experiments (NGF concentration range: 0.1–100 nM).

抗体-抗原结合亲和力

表面等离子体共振分析

Figshare2015-12-03 更新00

Summary of the epitope coverage and binding affinities of 31 anti-PD-1 mAbs.

抗PD-1抗体表位分析

抗体-抗原结合亲和力

(KD values represent the mean values from 8–12 spots per mAb, as determined on the LSA using a CMD-P chip; ND = not determined; kd-limited in SPR capture-based assay in bold). Data for mAb33 and mAb34

NIAID Data Ecosystem40

SPR measurements.

生物分子相互作用分析

抗体-抗原结合亲和力

Binfoding affinities between Fab5H2 and NHBA variants p2, p3 and p20 determined by Surface Plasmon Resonance.

Figshare2018-08-22 更新20