Transcriptional profiling of hepatocyte, reprogrammed hepatocyte, and biliary cells

Following liver injury, a subset of hepatocytes adopt features of biliary epithelial cells (BECs) in a process known as biliary reprogramming. The aim of this study is to elucidate the molecular events accompanying this dramatic shift in cellular identity. Wild type hepatocytes, wild type biliary cells, DDC-treated hepatocytes, DDC-treated biliary cells, and DDC-treated reprogrammed cells were isolated by FACS, RNA isolated, and sequenced. Transcriptional profiling of liver cell populations by RNA-seq in control and DDC-treated mice