Whole genome sequencing of Adaptive Laboratory Evolved Acinetobacter baylyi ADP1 strains

In this study, we actively engaged in the evolutionary adaptation of Acinetobacter baylyi ADP1 on five distinct synthetic lignin-derived monomers (LDMs), specifically p-coumaric acid, 4-hydroxybenzoic acid, caffeic acid, ferulic acid, and vanillic acid. Consequently, these adapted strains were designated as SAG_Cm, SAG_Hb, SAG_Cf, SAG_Fe, and SAG_Vn. Additionally, we conducted the co-evolution of a consortium comprising these independently evolved strains in a mixture containing all the above-mentioned LDMs, denoted as SAG_185. Likewise, we subjected the crc mutant of ADP1 to evolution in the synthetic lignin-derived monomer mixture, designated as SAG_186. We conducted comprehensive whole-genome sequencing on the original and evolved strains to pinpoint the beneficial mutations responsible for the improved growth in their respective LDMs. The primary objective of this study is to identify potential targets within the ADP1 genome for strategic metabolic engineering, ultimately paving the way for the development of a versatile platform strain in the field of lignin valorization.