BHCL_DMPC_Ca

This dataset contains NMR spectra obtained for the sample -BHCL_DMPC_Ca Nucleus: 13C NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9859 Observed Frequency: 125.78746 Magnetic Field Strength: 11.747332789100614 Number of Scans: 2048 NMR Pulse Sequence: NEA_ineptrd Spectral Width: 180.666405933068 Number of Data Points: 2270 Relaxation Delay: 6 Observed Frequency: 125.7968940595 Nucleus: 1H NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 296.0007 Observed Frequency: 500.248 Magnetic Field Strength: 11.749122218330188 Number of Scans: 32 NMR Pulse Sequence: onepulse Spectral Width: 19.9900065723262 Number of Data Points: 3006 Relaxation Delay: 5 Observed Frequency: 500.24996059 Nucleus: 31P NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: 1d Temperature: 295.9824 Observed Frequency: 202.504102 Magnetic Field Strength: 11.745385617451017 Number of Scans: 256 NMR Pulse Sequence: NEA_zg30bbdec Spectral Width: 201.284167395372 Number of Data Points: 5702 Relaxation Delay: 6 Observed Frequency: 202.504102 Nucleus: 31P NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: 1d Temperature: 295.9955 Observed Frequency: 202.504102 Magnetic Field Strength: 11.745385617451017 Number of Scans: 2048 NMR Pulse Sequence: NEA_zg30bbdec Spectral Width: 201.284167395372 Number of Data Points: 4072 Relaxation Delay: 6 Observed Frequency: 202.504102 Nucleus: 13C,1H NMR Solvent: H2O+D2O NMR Probe: H8609_0043 (PH MAS 500WB BL4 X/Y/H DVT) NMR Pulse Sequence: null Temperature: 295.9883 Observed Frequency: 125.78746,500.248 Magnetic Field Strength: 11.747332789100614 Number of Scans: 512 NMR Pulse Sequence: NEA_RPDLF Spectral Width: 180.666405933068,4.99752988789391 Number of Data Points: 2270,32 Relaxation Delay: 6 Observed Frequency: 125.7968940595,500.2471333

BHCL (10.2mg, 1.0 eq.), DMPC (20.0mg, 4.0eq.) and CaCl2 (0.3mg, 0.5eq.) were dissolved with
1ml chloroform:methanol (2:1, v/v) in an eppendorf vial. The solvent was evaporated at 40°C
under a constant stream of argon and the predried sample was freeze-dried over night. 23.15µl
degassed water was added and the Eppendorf was stored at 50°C for one day. Lost water was
added and the sample was mixed with ten freeze-thaw cycles (1min liquid N2 and 3min in 50°C
ultra sound bath). After another day of equilibration the sample was packed in the insert.