Choose Your Own Adventure: A Comprehensive Database of Reactions Catalyzed by Cytochrome P450 BM3 Variants

Cytochrome P450 BM3
monooxygenase is the topic of extensive
research
as many researchers have evolved this enzyme to generate a variety
of products. However, the abundance of information on increasingly
diversified variants of P450 BM3 that catalyze a broad array of chemistry
is not in a format that enables easy extraction and interpretation.
We present a database that categorizes variants by their catalyzed
reactions and includes details about substrates to provide reaction
context. This database of >1500 P450 BM3 variants is downloadable
and machine-readable and includes instructions to maximize ease of
gathering information. The database allows rapid identification of
commonly reported substitutions, aiding researchers who are unfamiliar
with the enzyme in identifying starting points for enzyme engineering.
For those actively engaged in engineering P450 BM3, the database,
along with this review, provides a powerful and user-friendly platform
to understand, predict, and identify the attributes of P450 BM3 variants,
encouraging the further engineering of this enzyme.