Autism spectrum disorder associated chromatin modifiers converge on transcription with sex-specific regulatory signatures

We sought to understand the transcriptional disruptions and functional impacts of the loss of 9 autism spectrum disorder (ASD) risk genes that encode transcriptional regulators in neurons. In addition to understanding how these signature converge or diverge, we aimed to study how sex could modulate these consequences. Overall design: Neurons were cultured from wildtype e16.5 mouse embryos seperated by males and females. Cultures were infected with lentivirus containing shRNA against a nontargetted control or each of 9 genes to deplete targets. Neurons were lysed at day in culture 10 to collect total RNA. RNA was reverse transcribed and RNA-seq libraries were prepared. (n = 3 per condition, per sex)