ZFP-1 and RDE-4 regulate transcription of pdk-1.

(A) ZFP-1::FLAG ChIP with anti-FLAG antibodies in wild type and rde-4(ne299) demonstrating DNA enrichment in IP relative to input by qPCR on the promoters – “P” and coding regions – “C” of the genes shown. Results of two biological replicas are shown; error bars represent Standard deviation. (B) pdk-1 mRNA levels measured by RT-qPCR in the drh-3 mutant at different larval stages and normalized to wild type. (C, D) RNA polymerase II ChIP with 8WG16 antibodies (Covance) in wild type and zfp-1(ok554) (C) or wild type and rde-4(ne299) (D) demonstrating DNA enrichment relative to wild type by qPCR on the promoters – “P” and coding regions – “C” of the genes shown. Results of two biological replicas are shown; error bars represent Standard deviation; * indicates significance of PFigure 2A. (E) pdk-1 pre-mRNA levels measured by RT-qPCR in indicated mutants (L4 stage animals) and normalized to wild type. Results of two biological replicas are shown; error bars represent Standard deviation.