scRNA-seq of brain glioblastoma sample

Proneural-mesenchymal transition (PMT) is a phenotypic alteration and contributes to therapeutic resistance and recurrence of glioblastoma (GBM). Macrophages, as a main infiltrating component of tumor immune microenvironment (TIM), can regulate the biological processes of PMT. However, the mechanisms driving this process remain largely unknown. Here, We performed single-cell RNA sequencing (scRNA-seq) (3V3) and Spatial transcriptomics RNA sequencing(stRNA-seq)(1V1) from tumor core and matching tumor periphery samples to discripe the overall landscape of tumor and nontumor cells in gliomas. Overall design: We profiled freshly isolated cells from tumor core and paired surrounding peripheral tissue in glioma patients. scRNA-seq (3V3) and stRNA-seq(1v1) were used to discripe the overall landscape of tumor and nontumor cells in gliomas.