Characterisation of ER-beta functions in prostate cancer (RNA-Seq)

To investigate the effect of a novel ER-beta selective ligand alone and with Enzalutamide on RNA expression in prostate cancer cell lines Overall design: LNCaP, LNCaP C4-2 and 22Rv1 cells in triplicate were treated with either vehicle, OSU-ERb-12 alone (100 nM) or E2 alone (100nM), Enza (1 microMolar) or the combinations for 12h and RNA-Seq was undertaken. . Sequencing libraries prepared with the TruSeq Stranded Total RNA kit (Illumina Inc), from 1ug total RNA. Alignment of raw sequence reads to the human transcriptome (hg38) was performed via Rsubread and transcript abundance estimates were normalized and differentially expressed genes (DEGs) identified using a standard edgeR pipeline.