Image Data and Analysis Materials for In-situ Fracture Mechanics Experiments on Human Cortical Bone Treated Ex-vivo with Raloxifene

<p>This publication documents 3D image stacks  for two in-situ loaded fracture mechanics specimens observed with 3D X-ray microscopy, 2D image stacks of the crack mouth opening displacement during said loading, and analysis codes which supported the publication.</p>

<p><strong>Materials</strong></p>

<p>The diaphysis of a human (75-year-old, male) cadaveric femur was obtained through the Indiana University School of Medicine Anatomical Donation Program. Nominal 4 mm x 4 mm x 24 mm beams were sectioned from the femur diaphysis. Experiments were conducted on 12 beams. Beams were assigned to 2 groups: treated with Raloxifene (RAL), treated with a vehicle (VEH) control. Image data are provided for one beam from the RAL group and one beam from the VEH group. Additional images can be made available upon request to the corresponding author. </p>

<p><strong>Imaging</strong></p>

<p>For in-situ fracture mechanics experiments:</p>

<p>2D and 3D scans were acquired by Glynn Gallaway using a 4-point bending rig for single edged notched bend specimens in a water bath with a Deben CT5000N load cell (Deben, Bury St. Edmunds, UK) in a Zeiss XRADIA 510 Versa 3D X-Ray microscope (Carl Zeiss AG, Baden-Württemberg, Germany) at Purdue University. The 4-point bending frame had a span 16 mm with X-ray transparent, glassy carbon supports. To maintain hydration, bending frame was situated in a waterbath filled with DI water. Displacements were applied at 0.1 mm/min. Load cell outputs were monitored and recorded. During loading 2D images were obtained every 1 second. 3D X-ray images were acquired with a resolution of 4.5 um, exposure time 6 sec., 2401 projections, 120 kV, 10 W, 4 x objective, and a LE2 filter. X-ray projections were processed through XRADIA Scout-and-Scan Reconstructor. A recursive Gaussian smoothing filter (s=1 pixel) was applied to reduce image artifacts. Image stacks are exported as tiff files and provided for each specimen. Images are provided for one RAL treated sample and one VEH treated sample.</p>