Single-cell RNA sequencing of human peripheral blood mononuclear cells reveals distinct immune response in gout flare and remission

Gout typically presents as an acute, self-limiting inflammatory monoarthritis. Based on single-cell RNA sequencing (scRNA-seq), we profiled peripheral blood mononuclear cells (PBMCs) from the same patients with gout flare and gout remission We identified the dynamics of cell abundance, gene expression patterns and biological processes underlying the immune dysregulation of each cell compartment. The single-cell landscape of both innate and adaptive immune responses provides new insights into pathogenesis and therapy of gout flare. PBMCs from the same patients with gout flare and gout remission were subjected to scRNA-seq with 10x Genomics and validated by flow cytometry and LC-MS/MS.