Multi-modal single-cell sequencing identifies cellular immunophenotypes associated with juvenile dermatomyositis disease activity

Juvenile dermatomyositis (JDM) is a rare autoimmune condition with insufficient biomarkers and treatments, in part, due to incomplete knowledge of the cell types mediating disease. We investigated immunophenotypes and cell-specific genes associated with disease activity using multiplexed RNA and protein single-cell sequencing applied to PBMCs from 4 treatment-naïve JDM (TN-JDM) subjects at baseline, 2, 4, and 6 months and 4 subjects with inactive disease. This study includes single-cell RNA and ADT (protein) measurements of PBMCs from 4 treatment-naïve JDM (TN-JDM) subjects at baseline, 2, 4, and 6 months and 4 subjects with inactive disease. Samples were multiplexed across four 10X reactions. Provided in GEO are the filtered RNA and ADT matrices we used for clustering and differential gene expression analysis. We have also included a metadata file with cell annotations and file with UMAP coordinates to re-create our cell clusters. >>> Submitter declares that the raw data will be deposited in dbGaP due to patient privacy concerns. <<<