CD99 intensity levels on MSC-P as assessed by Multiparameter Flow Cytometry.

All samples were positive for CD99 with low to moderate levels of intensity. Samples were analyzed at low passages (1–3). Primary tumors were located in distinct locations and all presented EWSR1 gene rearrangements and EWS-FLI 1 fusion in some cases. This situation was analyzed with comparison to a control which is the median of all tubes without antibody and intensity levels were calculated with ration between sample/control. (ratio2 +, ratio>10 ++, ratio>100 +++).1MFI stands for Median Fluorescence Intensity,2PN stands for Passage Number;3BA stands for Break Apart;4MD stands for Metastasis at diagnosis.