Thermodynamic parameters for the binding of full-length human PDI and its fragments to human Tau fragment Tau_244–372 (or full-length human Tau) as determined by ITC at 25.0°C.

Thermodynamic parameters, Kd, and n, were determined using a single set of identical sites model. The standard molar binding free energy () and the standard molar binding entropy () for the binding reaction were calculated using Equations 2 and 3 respectively. The buffer used was 10 mM HEPES buffer (pH 7.4) containing 100 mM NaCl. Errors shown are standard errors of the mean. NB, no binding observed in the present conditions. aThermodynamic parameters for the binding of full-length human PDI and its a-domain to Tau244–372 as determined by ITC at 37.0°C. bThermodynamic parameters for the binding of full-length human PDI to full-length human Tau as determined by ITC at 25.0°C.