RNA-Seq analysis of Arabidopsis thaliana esr3-2 mutant versus wild-type (JC66)

A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity and identified the esr3-2 (enhanced stress response 3) mutant which also conferred increased resistance to the fungal pathogen Fusarium oxysporum. RNA-Seq was conducted to compare esr3-2 and wild-type (GSTF8 promoter::luciferase reporter line JC66 in Col-0 background) transcriptomes for altered gene expression.