Animal monitoring data collected during multifaceted experiments from a sheep model being treated for smoke-induced acute lung injury using veno-venous extra corporeal membrane oxygenation

The source of data for this study was from a sheep model being treated for smoke-induced acute lung injury using veno-venous extracorporeal membrane oxygenation, a form of extracorporeal life support (ECLS) developed to complement the treatment of acute lung injury in humans. During this type of ECLS, venous blood is carried from the patient to a gas exchange device where it becomes enriched with oxygen, has carbon dioxide removed, and is returned to the patient’s circulation in the right heart. This method can be used for treatment, as respiratory support during lung transplantation, and in critically ill patients with potentially reversible respiratory failure.
The source study was an ongoing publicly funded animal experimentation study (Queensland University of Technology Animal Ethics Approval No. 110000053).
The overall goal of the study was to provide useful information relevant to the sheep model, itself, and to those interested in large animal experimentation and veterinary medicine, generally. The specific objectives were: 1) use the raw data from the sheep model study to create a data management system for tabulating large data sets from human studies using animal models and, 2) analyse that data to provide biological information that is not currently available for sheep receiving ECLS following smoke-induced acute lung injury.
Data from 19 adult mechanically ventilated ewes undergoing intensive care in a previous study evaluating a form of extracorporeal life support (treatment) for acute lung injury were used to develop a comprehensive method for processing manual and electronically gathered clinical observations. Eight sheep were injured by acute smoke inhalation prior to treatment (injured/treated), while another eight were not injured but treated (uninjured/treated). Two sheep were injured but not treated (injured/untreated), while one received room air instead of smoke as the injury, and was not treated (placebo/untreated). The data were then analysed for 11 physiological categories and compared between the two treated groups.
Data consisted of separate files of real-time physiological data recorded on the hard drives of the monitoring devices (electronically acquired data), and parameters manually recorded by those monitoring the sheep under anaesthesia (manually acquired data), which included data from the electronic monitoring equipment, as back-up if the electronic monitor malfunctioned.
Data were tabulated and processed using spreadsheet (Microsoft® Excel 2010, Microsoft Corporation).
To meet the second objective, data from the groups, uninjured/treated and injured/treated groups were analysed. The means, medians and standard deviations of the weights of the sheep, where applicable, were tabulated. The physiological parameters of the groups were charted and compared against each other using one-way analysis of variance (ANOVA), where appropriate. Parameters between groups were compared using a paired two-tailed t-test. All p-values were two-sided and p < 0.05 was considered statistically significant. All statistical calculations were performed using GraphPad PRISM 6 software (GraphPad Software, La Jolla, CA, USA).

本研究的数据来源为一项针对使用静脉-静脉体外膜肺氧合（一种为补充人类急性肺损伤治疗而开发的体外生命支持技术）对烟雾诱导的急性肺损伤进行治疗的羊模型的研究。在此类体外生命支持过程中，患者的静脉血液被输送到气体交换装置，在那里它被富含氧气，二氧化碳被移除，并通过右心返回患者循环。该方法可用于治疗，作为肺移植期间的呼吸支持，以及在可能可逆的呼吸衰竭的危重患者中。源研究是一项持续进行的由公共资金资助的动物实验研究（昆士兰科技大学动物伦理审查批准号110000053）。研究的总体目标是提供与羊模型本身及其对大型动物实验和兽医医学感兴趣者相关的重要信息。具体目标包括：1）利用羊模型研究的原始数据创建一个数据管理系统，用于整理来自使用动物模型的人类研究的庞大数据集，2）分析该数据以提供目前尚未对接受体外膜肺氧合治疗的烟雾诱导的急性肺损伤的羊的生物信息。19只成年机械通气的绵羊的数据被用于开发一种处理手动和电子收集的临床观察的综合方法。其中八只羊在治疗前因急性烟雾吸入而受伤（受伤/治疗），而另外八只未受伤但接受了治疗（未受伤/治疗）。两只羊受伤但未接受治疗（受伤/未治疗），其中一只羊在受伤时接受的是室内空气而非烟雾，并未接受治疗（安慰剂/未治疗）。随后，对两组治疗后的数据进行11个生理类别的分析，并进行比较。数据包括来自监控设备硬盘上记录的实时生理数据（电子获取数据）和那些在绵羊麻醉下监控它们的人员手动记录的参数（手动获取数据），其中还包括电子监控设备的数据，作为电子监控器出现故障时的备份。数据使用电子表格（Microsoft® Excel 2010，微软公司）进行整理和处理。为了实现第二个目标，对未受伤/治疗组和受伤/治疗组的组数据进行分析。在适用的情况下，整理了羊的体重平均值、中位数和标准差。使用单因素方差分析（ANOVA）将各组生理参数绘制成图表并进行比较，在适当的情况下，使用配对双尾t检验比较组间参数。所有p值均为双尾，p < 0.05被认为具有统计学意义。所有统计计算均使用GraphPad PRISM 6软件（GraphPad软件，美国加利福尼亚州拉霍亚）进行。