The underlying mechanism by which SFRS8 exerted carcinogenesis and its potential targets in MM

An RIP experiment was performed in ARP1 cell following the instructions of the article.~5-20 × 10e6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5µg of the antibody of interest (SFRS8, abcam) and incubated with cell lysate supernatant overnight at 4°C.The beads containing immunoprecipitated RNA-protein complex were treated with 150µL of Proteinase K buffer to digest the proteins. Specific binding RNAs were isolated by using TRIzol Overall design: Examination in ARP1 cells.