Markers of oxidative stress (DCF fluorescence and MDA levels) and metabolic response to an oxidative stimulus (t-BHP) were analyzed in lymphoblasts from ALS patients and matched controls.
Lymphoblasts from ALS patients and matched controls were seeded at 300000 cells (time 0) and cultured up to 96 h. Cells were counted at 24, 48, 72 and 96 h.
Summary of changes observed in lymphoblasts from ALS patients with mutant SOD1 (mutSOD1) or unknown mutation (undSOD1) and respective controls. Cell lines, antibodies and primers used for mitochond