Digital holograms of isolated HL60 cell nuclei

Digital holograms of isolated HL60 cell nucleiThese data were originally used by Schürmann et al. [1] for thequantification of the refractive index of isolated HL60 cell nuclei.Raw dataThe data are related to [1] according to the following scheme(last part before suffix indicates cell cycle stage):Figure 5 - 1st measurement:2014-05-29_DHM_HL60-nuclei-G1.zip2014-05-29_DHM_HL60-nuclei-G2.zip2014-05-29_DHM_HL60-nuclei-S.zipFigure 5 - 2nd measurement:2014-07-03_DHM_HL60-nuclei-G1.zip2014-07-03_DHM_HL60-nuclei-G2.zip2014-07-03_DHM_HL60-nuclei-S.zipData analysisData analysis in [1] has been performed largely manual. In [2],the analysis has been revisited with DryMass [3] which enables anautomated data analysis. The .cfg files are DryMass configurationfiles [4], i.e. analysis recipes that allow a complete data analysisfrom scratch. The file "HL60nuc.cfg" contains the basic data analysisrecipe. The file "ignore_ROIs.txt" contains the ignored regions ofinterest for each measurement. The other .cfg files contain the sameinformation with default values filled in by DryMass. The analysishas been tested with DryMass version 0.10.0.To run an analysis, issue e.g. the following command: dm_analyze_sphere -p 2014-05-29_DHM_HL60-nuclei-G1.cfg 2014-05-29_DHM_HL60-nuclei-G1.zipReferences[1] M. Schürmann et al., "Cell nuclei have lower refractive index and mass density than cytoplasm," Journal of Biophotonics 9(10): 1068–1076, 2016. doi:10.1002/jbio.201500273.[2] P. Müller et al., "DryMass: handling and analyzing quantitative phase microscopy images of cell-sized objects," manuscript in preparation.[3] https://drymass.readthedocs.io/[4] https://drymass.readthedocs.io/en/stable/sec_gs_configuration_file.html