Examining age-dependent DNA methylation patterns and gene expression in the male and female mouse hippocampus

We performed reduced representation bisulfite sequencing (RRBS) from mouse dorsal hippocampus – which is necessary for the formation and consolidation of specific types of memories – in young and aging mice of both sexes to determine age-dependent changes in methylation across genomic contexts. Overall, our findings demonstrate that methylation levels within the dorsal hippocampus are divergent between sexes during aging in genomic features correlating to mRNA functionality, transcription factor binding sites - with hypomethylated regions correlating with transcription factors involved in plasticity, and gene regulatory elements. DNA methylation analysis, including across context DMRs and histone modifications, of young and aging dorsal hippocampal mouse tissue