Identification of p85beta- or BCLAF1-enriched genomic regions

p85beta of the PI3K can localize to the nucleus. Our immunoprecipitation data showed that it physically interacts with a transcription factor BCLAF1. To reveal the enrichment of p85beta and BCLAF1 at genomic regions and to determine whether p85beta and BCLAF1 co-occupy at genomic regions, chromatin immunoprecipitation sequencing (ChIP-seq) was performed. Overall design: Chromatin harvested from DOV13 cells stably overexpressing HA-tagged p85beta was immunoprecipitated with either ChIP grade anti-HA antibody or anti-BCLAF1 antibody. Input DNA samples were included for background signal. Three biological replicates per condition.