Sodium azide mutagenesis induces a unique pattern of mutations

This dataset contains genomic variant calls, structural variation data, and phenotypic measurements related to Hordeum vulgare (barley). It includes VCF and BED files from multiple sequencing platforms—10x Genomics, Oxford Nanopore, PacBio, and Illumina featuring raw, filtered, and phased variants. Structural variant calls in BEDPE format and callable/un-callable region lists are provided. Phenotype data from field trials provide spatially adjusted and raw trait values for agronomic characteristics.

These data provide insight on genomic diversity, structural variation, and genotype-phenotype associations in barley. The use of multiple sequencing technologies enables cross-validation of variant calls, allowing for high-confidence genome annotation and population genetics studies. The filtered VCFs help isolate biologically relevant mutations, while the callable region data ensure rigorous quality control for variant interpretation.

This dataset is being released as it is supporting the associated paper to this research and also to support the broader research community in comparative genomics, evolutionary biology, and crop improvement studies. By making these resources publicly available, we aim to enhance reproducibility, enable novel insights into barley’s genomic architecture, and assist breeding efforts focused on climate resilience and agronomic performance.