Additional file 1 of Accelerated immune ageing is associated with COVID-19 disease severity

Additional file 1: Supplementary Figure 1. The long-term impact of COVID-19 on CD8 T cell subset distribution. Comparison of circulating numbers of: (A) CD8 T cells; (B) Naïve; (C) Memory; (D) Central memory; (E) Effector memory; (F) EMRA CD8 T cells between healthy age and sex matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients. If not indicated, p value is not significant. Supplementary Figure 2. The long-term impact of COVID-19 on CD4 T cell subset distribution. Comparison of the systemic numbers of: (A) CD4 T cells; (B) Naïve CD4 T cells; (C) Memory CD4 T cells; (D) Central memory CD4 T cells; (E) Effector memory CD4 T cells; (F) EMRA CD4 T cells in healthy age and sex-matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 survivors 3-5 months post-infection. Statistical analysis by two-sided Mann–Whitney non-parametric test. If not indicated, p-value is not significant. Supplementary Figure 3. CD4 T cell senescence and exhaustion post-COVID-19. Comparison of: (A) absolute numbers of CD28-veCD57+ve CD8 T cells in healthy age and sex matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients; (B) percentage of CD28-veCD57+ve CD4 T cells in (n = 59) and mild (n = 15), moderate (n = 29) and severe (n = 55) COVID-19 survivors 3-5 months post-infection; (C) absolute numbers of CD28-veCD57+ve CD4 T cells in healthy age and sex matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients; (D) absolute numbers of KLRG1+ve CD8 T cells in healthy age and sex matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients; (E) percentage of KLRG1+ve CD4 T cells in (n = 59) and mild (n = 15), moderate (n = 29) and severe (n = 55) COVID-19 survivors 3-5 months post-infection; (F) absolute numbers of KLRG1+ve CD4 T cells in healthy age and sex matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients. (G) percentage and (H) absolute numbers of PD1+ve CD4 T cells in healthy age and sex-matched controls (n = 21) and severe (n = 18) COVID-19 convalescent patients. Statistical analysis by two-sided Mann–Whitney non-parametric test. If not indicated, p-value is not significant. Supplementary Figure 4. B cell subsets post-COVID-19. Absolute numbers of systemic: (A) memory B cells; (B) plasma B cells; (C) regulatory B cells in healthy age and sex-matched controls (n = 39), moderate (n = 14) and severe (n = 46) COVID-19 convalescent patients. Statistical analysis by two-sided Mann–Whitney non-parametric test. If not indicated, p-value is not significant.