Comparative transcriptome analysis on Arabidopsis thaliana treated by guvermectin, gibberellin acid 3, trans-zeatin, indole-3-acetic acid or epibrassinolide

Purpose: To compare transcriptomic changes after guvermectin (GV), indole-3-acetic acid (IAA), trans-zeatin (ZT), epibrassinolide (eBL) and gibberellin acid 3 (GA3) treatments in Arabidopsis. Method: 7-day-old MS liquid cultivated Col-0 seedlings were treated by 10 μM GV, 1 μM IAA, 1 μM ZT, 1 μM eBL or 1 μM GA3. Chemicals were added into MS liquid respectively and whole seedling samples were collected after treatment for 5, 30, 180 minutes. In total, we got control group and 5 treatment groups. Three biological replicates were performed. Results: In total, 54 samples were used for RNA sequencing analysis. At least 6 G clean bases were generated for each sample. Comparative analysis identified differences between GV and other plant growth regulators in transcriptional level. In this study, transcriptome changes after 10 μM GV, 1 μM IAA, 1 μM ZT, 1 μM eBL or 1 μM GA3 treatment were compared at 5, 30, 180 minutes. Whole seedling samples from control and treated plants were collected for RNA isolation. 18 treatments in triplicate were used in this study.