Analysis of DSB repair pattern in A. thaliana wss1A mutants

In order to test whether WSS1A plays a direct role in DSB repair, we induced site-specific DSB in the mutant as well as in WT Arabidopsis thaliana plants using the CRISPR/Cas system. To avoid enzyme- or locus-specific effects, we induced breaks at three different loci located in eu- and heterochromatic regions with two different nucleases, SpCas9 and SaCas9. Our analysis revealed no repair deficiency in wss1A mutant plants. Also, no changes in pathway choice were detectable for any locus. Thus, WSS1A is neither involved in replication unrelated DSB repair itself nor influencing its outcome.