E96A baseline transcriptional changes

APE1/Ref-1 is the primary apurinic/apyrimidinic endonuclease in the base excision repair pathway. To define baseline transcriptional changes resulting from loss of APE1 endonuclease activity, RNA-seq was performed on Pa03C pancreatic ductal adenocarcinoma cell lines engineered to carry a homozygous E96A mutation and on matched Cas9 controls. Cells were cultured under untreated conditions. This dataset provides gene expression profiles associated with impaired APE1 endonuclease function in PDAC cells. Overall design: Pa03C pancreatic ductal adenocarcinoma (PDAC) cell lines were engineered to carry a homozygous APE1 E96A endonuclease-deficient mutation or a matched Cas9 control allele. Cells were cultured under standard growth conditions without drug treatment. RNA-seq was then performed on Pa03C Cas9 and Pa03C E96A PDAC cell lines under untreated conditions. The study aims to capture baseline transcriptional consequences of impaired APE1 endonuclease activity