Fluorescent Membrane Tension Probes for Early Endosomes

Table of contents 1. Synthesis and Characterization 1.1. Compounds 1, 3, 4, 6, 7, 9, 10, 11, 12 Lab Book Page NMR LC-MS IR HRMS 1.2. Compounds 15, 17, 19, 24, 1’, 3’, 5’, 7’ Lab Book Page NMR LC-MS IR ESI-MS 1.3. Compounds 21, 23, 26, 8’, 10’ Lab Book Page NMR LC-MS IR 2. pKa Determination 2.1. Titration of 32, 35, 36 for the determination of the correction function Lab Book Page NMR 2.2. Titration of Model Compounds 1’ – 10’ Lab Book Page NMR 3. Co-Localization with EGF 4. Automated Microscopy 5. FLIM Imaging 6. FLIM of Early Endosomes