Brachypodium distachyon transcriptome of distinct developmental stages. Brachypodium distachyon

In this study, we explored landscape of AS events in Brachypodium distachyon during juvenile, adult and reproductive transitions. Moreover, we revealed that SCL33, a serine/arginine-rich splicing factor in spliceosomes, plays a redundant and antagonistic role with its paralog, SCL33L, in regulation of intron assembly across distinct developmental stages. In addition, we determined global AS variations in miR156 overproducing plants, and interestingly found that SPLs were able to be regulated by miR156 in intron retention alteration besides mRNA clearance and translation repression manners. Finally, we demonstrated a complex regulatory process of age-dependent AS events in Brachypodium, which were coincidently or separately regulated by miR156 and SCL33/SCL33L. These results together illustrate that dynamic regulations of AS contribute significantly to growth transitions in plants.