Interferon-? couples CD8+ T cell avidity and differentiation during infection

Expression and relevance of IFNg signaling in CD8 T cells during infection Overall design: Expression profiling by high throughput sequencing. For 24hrs experiment, C57 Bl6 mice were left naive or infected with Listeria-OVA infected 24 hrs . CD3+ CD8+ T cells were isolated by FACS from the spleen and subjected to scRNAseq. For day 9 experiment, C57Bl6 WT mice and CD8-IFNgRKO mice were infected with LM-OVA. CD3+ CD8+ OVA-tetramer+ T cells were isolated by Fluorescence-activated cell sorting (FACS) from the spleen after 9 days and subjected to scRNA and scTCR sequencing.