Saliva methylome analysis of ART children that underwent embryo culture in G3 or K-SICM medium. Saliva methylome analysis of ART children that underwent embryo culture in G3 or K-SICM medium

Follow-up study of 9 year old IVF children who underwent embryo culture in G3 (Vitrolife) or K-SICM (Cook) medium. Genome-wide DNA methylation profiling of 9 year old IVF children (saliva samples) who had undergone embryo culture in G3 medium (Vitrolife) or K-SICM medium (Lonza). The EPIC array was used to profile the methylome at approximately 850,000 CpG sites across the human genome. Overall design: Bisulphite converted DNA from umbilical cord blood samples from IVF 9-year-olds that had undergone embryo culture in G3 (n = 57 ) or HTF (n = 49) culture medium, were hybridised to the Illumina EPIC array. Data processing was conducted in R using the RnBeads package. Processing consisted of SWAN normalisation, and site/sample removal based on greedycut (detection p-value threshold 0.05), SNP proximity, position on the sex chromosomes, missing values in >5% of samples and sites not in a CpG context.