WGS sequencing, assembly, and annotation

The Department of Energy (DOE) Joint Genome Institute (JGI), in collaboration with the USDA - Agricultural Research Service and Plant Research International, sequenced the Mycosphaerella graminicola strain IPO323 genome at 8.9X coverage using whole genome shotgun (WGS) sequencing. The genome was annotated using both automated and manual methods. The M. graminicola genome is being finished at the Stanford Human Genome Center (SHGC). The M. graminicola isolate IPO323 is a Dutch field strain that was isolated in 1984 from the wheat cultivar Arminda.

美国能源部（DOE）联合基因组研究所（JGI）与美国农业部-农业研究服务局及植物研究国际组织共同协作，采用全基因组鸟枪法（WGS）测序技术，以8.9倍覆盖率对小麦叶点霉（Mycosphaerella graminicola）菌株IPO323的基因组进行了测序。该基因组注释采用了自动化与人工相结合的方法。小麦叶点霉基因组正在斯坦福人类基因组中心（SHGC）进行精细构建。Mycosphaerella graminicola菌株IPO323为一荷兰田间分离株，于1984年从小麦品种Arminda中分离获得。