cDNA sequences of Arabidopsis thaliana Col-0 in response to a broad spectrum biocontrol agent, Burkholderia sp SSG

Plants applied with a biocontrol agent Burkholderia sp isolate SSG one week or longer before being challenged with pathogens remain moderately resistant against a broad spectrum of pathogens. This project analyzed the transcriptome of Arabidopsis Col-0 sprayed with and without SSG cell suspension to determine whether such protection may result from defense priming triggered by the biocontrol agent. cDNA sequencing was done with Oxford Nanopore Technology for the SSG treated and mock plants 6 and 24 hours post-inoculation. The data analysis supports that SSG is an ISR inducer conferring plant protection upon pathogen encounter besides a pathogen suppressor and plant growth promoter previously described.

施用生物防治剂Burkholderia sp isolate SSG一周或更长时间后，在遭受病原体挑战的植物表现出对广泛病原体的中度抗性。本研究分析了经SSG细胞悬浮液喷洒的拟南芥Col-0转录组，以确定这种保护是否可能源于生物防治剂触发的防御启动。对经SSG处理的植物和模拟植物在接种后6小时和24小时使用牛津纳米孔技术进行cDNA测序。数据分析结果表明，SSG不仅是一种病原体抑制剂和植物生长促进剂，如前所述，还是一种ISR诱导剂，能够在植物遭遇病原体时赋予植物保护。