Genome-wide analysis of LY294002 effect on the glucocorticoid receptor function in human keratinocytes

LY294002 increased negative effect of glucocorticoid fluocinolone acetonide (FA) on gene expression and blunted activation of some GR-target genes by glucocorticoid FA. HaCat cells grown in Phenol free DMEM supplemented with 10 % Charcoal stripped Fetal Bovine Serum and 1 X Pen/Strep a day prior to the experiment were either pre-treated with 50 μM LY294002 for 6 h followed by 1 μM of FA for additional 24 h (IB4 and IB8) or were treated with 1 μM of FA alone for 24 h (IB2 and IB6). Control cells used for comparison were either treated with DMSO (vehicle control. IB1 and IB5) or 1 μM of LY294002 alone (IB3 and IB7). The experiments were performed in duplicates.