Raw data for fig 1B, S1B and S1C
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<b>Contents:</b> Combineddata06012014.mat accompanies Fig.1B, S1B and S1C of Sampattavanich et al., Cell Systems (2018). The file contains two variables: <i>reporter</i> and <i>parental</i>, each contains data from 5 timepoints: Each variables contain timepoint struct variables, each with 8x12: Time0: {8×12 cell};Time15: {8×12 cell};Time45: {8×12 cell};Time90: {8×12 cell};Time120: {8×12 cell}.<br>Each cell then contains the following data fields:<br> 'nuc_area' 'nuc_coordX' 'nuc_coordY' 'nuc_formfactor' 'nuc_integratedDAPI' 'nuc_integratedFoxO3' 'nuc_integratedWCDGreen' 'nuc_meanDAPI' 'nuc_meanFoxO3' 'nuc_meanWCDGreen' 'nuc_normmeanFoxO3' 'log10CoverN_extended_norm' 'log10CoverN_extended' 'log10CoverN_4pixel_norm' 'log10CoverN_4pixel' 'extendedcyto_area' 'extendedcyto_integratedDAPI' 'extendedcyto_integratedFoxO3' 'extendedcyto_integratedECDGreen' 'extendedcyto_meanDAPI' 'extendedcyto_meanFoxO3' 'extendedcyto_meanWCDGreen' 'smallcyto_area' 'smallcyto_integratedDAPI' 'smallcyto_integratedFoxO3' 'smallcyto_integratedWCDGreen' 'smallcyto_meanDAPI' 'smallcyto_meanFoxO3' 'smallcyto_meanWCDGreen'<br><b>To use this data with our Git Repository:</b>Placed these in the /rawdata/parentalVSReporter folder.<br> <b>Experimental Setup:</b><br> Treatment Condition: Cells were serum starved for 6 hours, then treated with combination of drugs and ligands for 0, 15, 45, 90, 135 minutes. Parental 184A1 cells were immunostained with FoxO3 antibody. Engineered 184A1 cells with F3aN400 sensor were fixed and directly imaged.<br>
提供机构:
Somponnat Sampattavanich
创建时间:
2018-05-09



