SARS-CoV-2 Sequencing

Successful antiviral drugs, in theory, exert their virus-specific effects by interacting with viral receptors, virally encoded enzymes, viral structural components, viral genes, or their transcripts without disturbing cellular metabolism or function. Although nirmatrelvir has been used for the treatment of SARS-CoV-2/COVID-19, some reports have been mentioned the emergence of drug-resistant-related amino acid substitutions. For elucidation of the mechanism of development of resistance to MPI such as NIR by SARS-CoV-2, it is critical to acquire MPI-resistant SARS-CoV-2 variant(s), which should be of high utility in further designing more efficacious and resistance-deferring anti-SARS-CoV-2 drugs.