Epigenetic and biochemical determinants of Pol IV-dependent genome surveillance in plants

Whole genome bisulfite sequencing (WGBS) and small RNA-seq datasets were generated from wild-type (Col-0) Arabidopsis thaliana plants and a panel of point mutants affecting the N-terminus of the largest subunit of RNA polymerase IV (Pol IV). Differentially methylated regions, small RNA clusters and transposable element activity observed in these genetic backgrounds were used to chart requirements for the NRPD1 N-terminus in Pol IV-dependent genome surveillance.