Transcriptome Investigation of HFD-fed mice treated or not with Glycoursodeoxycholic Acid

Recent studies reveal that bile acid metabolite composition and its metabolism are changed in metabolic disorders, such as obesity, type 2 diabetes and metabolic associated fatty liver disease (MAFLD), yet its role and the mechanism remain largely unknown. Hepatic whole transcriptome analysis identified glycoursodeoxycholic acid (GUDCA), glycine-conjugated bile acid produced from intestinal bacteria, differentially regulated 189 genes involved in biological processes including sterol, cholesterol and steroid biosynthesis, lipid metabolism, circadian rhythm and regulation, and the cellular component in cytosol, cytoplasm and endoplasmic reticulum, and molecular function including catalytic activity and platelet-derived growth factor binding. Overall design: Hepatic mRNA profiles of Vehicle-treated and GUDCA-treated mice receiving HFD diet (60% fat) (n=3/group).