Single-cell RNA-sequencing of tumors from PyMT;Shcbp1+/+ and PyMT;Shcbp1-/-mice

This study sought to investigate the role and mechanism of SHCBP1 in tumorigenesis of ductal carcinoma. We generate a global Shcbp1 knockout mice and introduced Shcbp1 deletion into MMTV-PyMT mice, which spontaneously develop mammary ductal tumors. We found that, compared with wild type (PyMT;Shcbp1+/+), Shcbp1 knockout (PyMT;Shcbp1-/- ) profoundly impedes tumor progression, metastasis and prolongs survival. We also found that Shcbp1 knockout-inhibited tumor progression is related to primary cilia. To confirm the possible connection between SHCBP1-deletion and primary cilia, we conducted single-cell RNA-sequencing (scRNA-seq) of tumors from PyMT;Shcbp1+/+ and PyMT;Shcbp1-/- mice, and analyzed the correlation between SHCBP1 knockout and ciliary signaling pathways.