Expression date from mouse Hepatocytes

The fields of drug discovery and regenerative medicine require large numbers of adult human primary hepatocytes. For this purpose, it is desirable to use hepatocyte-like cells (HLCs) differentiated from human pluripotent stem cells. To develop an efficient HLCs induction method, we constructed a red fluorescent reporter, CYP3A7R, in which DsRed is placed under the transcriptional regulation of CYP3A7 coding for a human fetus-type P450 enzyme. We created transgenic mice using mouse embryonic stem cells (mESCs) carrying a CYP3A7R transgene. CYP3A7R mESCs were used to optimize the HB-LC induction procedure. These induction conditions were applied to TT2F mESC and matured to HLC. To confirm the similarity of the highly expressed genes in these HLCs, Genechip analysis was used to compare them to transgenic mice FL and AL. The gene expression of the HLCs generated in this study was confirmed by comparison. Comparisons were made with HLCs (day-41 of differentiation), transgenic mouse fetal liver (E18.5: embryonic 18.5-day old fetus) and adult liver (6W: 6-week old mouse).