Perturb-Seq scRNA-seq for Alternative Promote Usage Sites
收藏NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP165596
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Using the Direct Capture Perturb-seq technology (Replogle, J.M. et al. Nat Biotechnol 38, 954â961 (2020)), we created a pooled of guides that target sites of promoter usage within 50 gene candidates on a stable MCF-7 KRAB dCas9 cell line. Single-cell sequencing 10x5' with CRISPR library and transcriptome. Sequenced on a Nova-Seq.
创建时间：
2025-11-20
