Perturb-Seq scRNA-seq for Alternative Promote Usage Sites

Using the Direct Capture Perturb-seq technology (Replogle, J.M. et al. Nat Biotechnol 38, 954–961 (2020)), we created a pooled of guides that target sites of promoter usage within 50 gene candidates on a stable MCF-7 KRAB dCas9 cell line. Single-cell sequencing 10x5' with CRISPR library and transcriptome. Sequenced on a Nova-Seq.